Extended Data Figure 9: Multiple sequence alignment of HODM including Aer2 PAS domain secondary structure information.
From: An oxidative N-demethylase reveals PAS transition from ubiquitous sensor to enzyme
Alignment of HODM proteins from the DUF3445 family from the bacteria: Pseudomonas mendocina (Pmen), Rhizobium etli (Retl), Candidatus Puniceispirillum marinum (Cpun) and Gordonia bronchialis (Gbro), and from the fungi Candida dubliniensis (Cdub) and Nectria haematococca (Nhae). The catalytic Arg, Glu and Gln residues and the substrate-discriminating Trp are marked with black dots. The conserved haem-binding His in Pmen and Aer2 are marked with a grey and a black asterisk, respectively. Secondary structural elements are highlighted, PAS-domain-specific α-helices in blue and β-sheets in pink, and HODM-specific α-helices in pale grey and β-sheets in dark grey.
