Extended Data Figure 6: Assessment of molecular pathway changes in response to modulation of In-PDGFRα levels.
From: Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis
a, Ingenuity pathway analysis of control-AMO-treated FAPs stimulated with PDGF-AA (50 ng ml−1) show enrichment for genes associated with fibrosis compared with control cells. b, Western blot showing phosphorylation of SMAD2/3 in response to PDGF-AA stimulation (50 ng ml−1). c, d, Gene set enrichment analysis (GSEA) of FAPs treated with the pA-AMOs compared to control-treated samples were analysed for enrichment of pathways in the Reactome (c) and BioCarta (d) databases30,31. Top sets with a false discovery rate of less than 5% are shown. e, The heat map displays a subset of genes that are upregulated with a false discovery rate of less than 0.1% in the pA-AMO- treated FAPs. The colours indicate the Z-score for expression normalized for each gene. Red, high expression; blue, low expression. f, g, Similarly, enrichment analyses of FAPs treated with the 5′ss-VMO compared to controls were performed using the Reactome (f) and BioCarta (g) databases30,31. h, A heat map displaying top upregulated genes in 5′ss-VMO-treated FAPs with parameters as specified in e is shown.
