Extended Data Figure 3: Phosphorylation of PDGFRα and downstream signalling components in FAPs in response to PDGF-AA stimulation is altered by changes in In-PDGFRα expression.
From: Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis
a, Western blot showing phosphorylation of PDGFRα, PLCγ, Akt and ERK1/2 in response to PDGF-AA stimulation. b, Quantification of data in a. c, Viral overexpression of In-PDGFRα in FAPs results in decreased signalling through Akt and ERK/2 whereas FL-PDGFRα levels remain constant. b, c, n = 3 biological replicates of pooled FAPs per condition except for the ERK1/2 condition in b, where n = 4. Error bars represent s.e.m. *P < 0.05; **P < 0.01; unpaired Student t-tests.
