Extended Data Figure 4: FAPs proliferate in response to PDGF stimulation.
From: Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis
a, EdU incorporation in FAPs stimulated with indicated amounts of PDGF for 20 h and incubated in EdU for the final 4 h. b, EdU incorporation in FAPs incubated with indicated amounts of PDGF and EdU for 24 h. c–f, Knockdown of In-PDGFRα by pre-treatment of FAPs with pA-AMOs (c) or In-PDGFRα-specific siRNA (In-siRNA) (d) increases FAP proliferation in response to PDGF-AA, whereas In-PDGFRα upregulation by 5′ss-AMO pre-treatment (e) or viral overexpression (f) decreases proliferation. For a, b, d, f, n = 3 biological replicates of pooled FAPs per condition. Error bars represent s.e.m. *P < 0.05; ****P < 0.0001; using unpaired Student t-tests.
