Extended Data Figure 5: Knockdown of In-PDGFRα and FL-PDGFRα with siRNAs.
From: Intronic polyadenylation of PDGFRα in resident stem cells attenuates muscle fibrosis
a, Schematic of the FL-PDGFRα transcript showing the location of siRNAs designed to knockdown In-PDGFRα (In-siRNA) and FL-PDGFRα (FL-siRNA). Arrows designate relevant polyadenylation sites. b–d, FL-siRNA induces transcriptional knockdown of FL-PDGFRα but not In-PDGFRα (b) and protein knockdown of FL-PDGFRα (c). At the same time, In-siRNA induces knockdown of In-PDGFRα (d). In b and d, n = 3 biological replicates of pooled FAPs per condition. The samples in b, and d, were all processed together so that the control condition in b is identical to the control condition in d. Error bars represent s.e.m. *P < 0.05, **P < 0.01; unpaired Student t-tests.
