Extended Data Figure 1: CCR2-T4L crystals and crystal packing.
From: Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists
a–c, Crystal packing of CCR2-T4L. CCR2 is a blue ribbon with ECL2 coloured red and T4L yellow. The unit cell is shown as a green box. CCR2-T4L molecules are arranged in a type I packing with hydrophilic stacking mediated by T4L and T4L–ECL2 interactions along axis c. a, Crystal packing in the ac plane. CCR2 makes abundant hydrophobic contacts with its neighbour via an interface mediated by antiparallel helix IV–helix VI interactions related by a screw axis along axis a. b, Crystal packing in the bc plane. Contacts between receptors and T4L involve ECL2 and the intracellular surface of CCR2 including helix VIII. Direct contacts between T4L are along axis b. One layer of CCR2-T4L molecules at the very top of the stacking column is omitted for clarity. c, Crystal packing in the ab plane. There are no direct interactions between T4L along axis a. d, Crystals of CCR2-T4L in the LCP bolus. Average crystals grew to 60 μm × 10 μm × 10 μm before harvesting.
