Extended Data Table 4 Observed association and dissociation rate constants of [3H]CCR2-RA (7 nM) on membranes from CHO cells transiently expressing WT CCR2 and CCR2-T4L, in the absence or presence of 1 μM BMS-681

From: Structure of CC chemokine receptor 2 with orthosteric and allosteric antagonists

  1. Values represent mean ± s.e.m. of three independent experiments performed in duplicate.
  2. †The percentage of maximum binding in the absence (Bcontrol) or presence (B) of BMS-681 (1 μM).
  3. ‡For CHO-CCR2-T4L only, dissociation kinetics of [3H]CCR2-RA (7 nM) in the presence of BMS-681 (1 μM) fitted best with a monophasic exponential decay model, resulting in a single koff value, as shown in the table. Thus, for CHO-CCR2-T4L, the statistical significance between koff measurements with and without BMS-681 could not be calculated.
  4. Statistical significance was analysed using a Student’s t-test, with significant differences versus control noted as follows: *P < 0.05, **P < 0.01, ****P < 0.0001.
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