Extended Data Figure 2: Spontaneous pulmonary metastases and primary tumour growth in Spns2 mice.
From: Genome-wide in vivo screen identifies novel host regulators of metastatic colonization
a, Size measurements of spontaneous pulmonary HCmel12–mCherry melanoma cell metastases of male mice with representative fluorescent images (lines indicate the edge of the lungs); n = 10 per genotype, horizontal bars represent mean (of 50 individual metastases counted per genotype) (one-way ANOVA with blocking factor of experiment, cumulative results of two independent experiments shown). b, Survival curve of +/+ and tm1a/tm1a male mice (n = 10 per genotype) in a spontaneous metastasis assay using HCmel12–mCherry cells (log-rank test (Mantel–Cox), cumulative results of two independent experiments shown). c, Growth of subcutaneously administered B16-BL6 cells in +/+ (four male, five female) and tm1a/tm1a (five male, one female) mice. Symbols represent mean ± s.e.m. with a two-tailed unpaired t-test with Welch’s correction used to compare the area under the curve. d, Incidence of cancer in aged (>40 weeks) +/+ (n = 15; 4 males, 11 females) and tm1a/tm1a (n = 18; 5 males, 13 females) mice. Statistical analysis was performed using a Fisher’s exact test.
