Extended Data Figure 5: Characterization of the phenotype of lymphatic endothelial cell Spns2 deficient mice.
From: Genome-wide in vivo screen identifies novel host regulators of metastatic colonization
a, b, Sphingoid base levels in the (a) serum or (b) lung of control and Spns2tm1c/tm1c; Lyve1cre/+ male mice (data are mean ± s.e.m., control n = 11, Spns2tm1c/tm1c; Lyve1cre/+ n = 10, multiple two-tailed unpaired t-tests with P value adjusted by the Holm–Šídák method with α set to 5%). Sph, sphingosine; DH-Sph, dihydrosphingosine; S1P, sphingosine-1-phosphate; DH-S1P, dihydrosphingosine-1-phosphate. c, Lymphocyte subsets in the spleen, lymph node, lung and liver of +/+ and tm1a/tm1a male mice (symbols represent individual mice, horizontal bars represent mean, multiple two-tailed unpaired t-tests with P value adjusted by the Holm–Šídák method with α set to 5%; data shown are representative of three independent experiments). d, Experimental metastasis assay using MC-38 cells in control (n = 9) and Spns2tm1c/tm1c; Lyve1cre/+ (n = 5) in female mice. Data shown are mean ± s.e.m., Mann–Whitney test, representative of three independent experiments.
