Extended Data Figure 5: Characterization of Piezo2 knockdown in Piezo2 conditional knockout mice.
From: Piezo2 senses airway stretch and mediates lung inflation-induced apnoea
a, Characterization of Piezo2 knockdown by qRT–PCR using FACS-sorted CD31+ (or PECAM1+) lung cells from adult wild-type and Tie2Cre;Piezo2cKO mice. b, Piezo2 in situ hybridization in the jugular–nodose complex of adult wild-type and Phox2bCre;Piezo2cKO mice. Dotted circles mark nodose ganglia (N). c, qRT–PCR using DRG isolated from P0 wild-type and Wnt1Cre;Piezo2cKO pups. *P < 0.05, ***P < 0.001, ****P < 0.0001, unpaired Welch’s t-test for a, c. Data are presented as mean ± s.e.m. Scale bar, 100 μm.
