Extended Data Figure 2: Piezo2 expression in the respiratory system.

a, Co-immunostaining for GFP and Nefh in P0 Piezo2^GFP reporter lung. Arrow, NEB. Smaller panels on right show a magnified view of NEB stained for GFP and CGRP (a marker of NEBs). b, c, GFP immunostaining in the jugular–nodose complex from P0 (b) and adult (c) Piezo2^GFP reporter mice. Dotted line demarcates the boundary of the jugular–nodose complex. d, e, GFP immunostaining in P0 trigeminal ganglion (d) or adult thoracic DRG (e) from Piezo2^GFP reporter mice. Dotted line in (d) demarcates the boundary of the trigeminal ganglion. f, Co-immunostaining for GFP and NeuN in a sagittal section of P0 Piezo2^GFP brainstem. g, h, GFP and αBTX (a marker of neuromuscular junction) co-staining in P0 Piezo2^GFP diaphragm (g) and intercostal muscles (h). i, Co-immunostaining for GFP and NeuN in adult Piezo2^GFP lumbar spinal cord. Dotted circle indicates motor neuron localization. j, Co-immunostaining for GFP and NeuN in adult Piezo2^GFP brainstem. Dotted circle marks dorsal nucleus of the vagus nerve, X. k, Co-immunostaining for GFP and Tuj1 in thoracic sympathetic ganglia from adult Piezo2^GFP reporter mice. l, Schematic summary of Piezo2–GFP expression in the respiratory system. VII, facial nucleus; BötC, the Bötzinger complex; VRG, ventral respiratory group; RB, rib bone; Vt, ventricle. Scale bars, 20 μm (smaller panels on right in a), 100 μm (all other panels).