Extended Data Figure 3: Characterization of tdTomato expression in the respiratory system of Piezo2-GFP-IRES-Cre (Piezo2GFP);Ai9 reporter mice.
From: Piezo2 senses airway stretch and mediates lung inflation-induced apnoea
a, b, Immunostaining for PECAM1 (a) and CGRP (b) with tdTomato epifluorescence in postnatal lungs. Arrow in (b) indicates tdTomato+ nerve fibre innervating NEB. Dotted line in (b) demarcates the lung epithelium. c, tdTomato epifluorescence in P0 jugular–nodose complex. Dotted line in (c) demarcates the boundary of the jugular–nodose complex. d, tdTomato epifluorescence in adult thoracic DRG. e, f, tdTomato epifluorescence in adult nucleus of the solitary tract (NTS) (e) and in adult spinal trigeminal nucleus (Sp5) (f), where axons of nodose and jugular/trigeminal sensory neurons project, respectively52,53. Smaller panels on right in f show a magnified view of Sp5 with Nefh staining. g–i, PECAM1 immunostaining with tdTomato epifluorescence in P0 brainstem (g), adult diaphragm (h), and P0 intercostal muscle (i). j, PV immunostaining with tdTomato epifluorescence in postnatal lumbar spinal cord. Dotted circle marks motor neuron localization. k, Tuj1 immunostaining with tdTomato epifluorescence in adult thoracic sympathetic ganglia. l, ChAT immunostaining and tdTomato epifluorescence in tracheal parasympathetic ganglia from adult reporter mice. Scale bars, 25 μm (a, l, smaller panels on right in f), 20 μm (b), 100 μm (c–k).
