Extended Data Figure 6: The roles of GATOR2 components in SZT2 regulation of mTORC1 signalling.

a–c, Cells were deprived of amino acids for 1 h and stimulated with amino acids for 10 min where indicated. Total cell lysates were analysed by immunoblotting. d, Control or SZT2-deficient cells were transfected with LentiCrisprV2 plasmids targeting GFP or SEH1L, selected with puromycin, and analysed as in a. Data (a–d) are representative of three independent experiments.