Extended Data Figure 8: Lysosome-targeted WDR59 or SESN2 suppresses mTORC1 signalling in GATOR1-deficient cells.

a, b, d, Cells were transfected with the indicated constructs and selected with puromycin. Cells were deprived of amino acids for 1 h and stimulated with amino acids for 20 min where indicated. Total cell lysates were analysed by immunoblotting. c, e, Cells were deprived of amino acids for 1 h and stimulated with amino acids for 20 min where indicated. Total cell lysates were analysed by immunoblotting. Data (a–e) are representative of three independent experiments.