Extended Data Figure 4: An intact SOG complex is essential for mTORC1 regulation.

a, HEK293T cells were transfected with the indicated constructs. Anti-Flag immunoprecipitates and total cell lysates were analysed by immunoblotting. b, SZT2-deficient HEK293T cells were transfected with the indicated constructs, selected with puromycin, and deprived of amino acids for 1 h. Total cell lysates were analysed by immunoblotting. c, d, HEK293T cells were transfected with the indicated constructs. Anti-Flag immunoprecipitates and total cell lysates were analysed by immunoblotting. e, SZT2-deficient HEK293T cells were transfected with the indicated constructs, selected with puromycin, and deprived of amino acids for 1 h. Total cell lysates were analysed by immunoblotting. f, A diagram of SZT2 protein and its truncation mutants, and a summary of their NPRL3 and WDR24 binding capacity. Data (a–e) are representative of three independent experiments.