Extended Data Figure 5: Impairment of cytosolic Hsp70 leads to enhanced import of unfolded proteins into mitochondria and mitochondrial damage.
From: Cytosolic proteostasis through importing of misfolded proteins into mitochondria
a, b, Representative images (a) and quantification (b) of split-GFP signal for FlucSM–GFP11 in the Δssa2 Δssa3 Δssa4 ssa1ts strain. Cells growing at 23 °C were treated with CHX for 30 min. Shown are mean and s.e.m. of 126 and 133 cells imaged and measured; three biological repeats. Unpaired two-tailed t-test: **P < 0.01. c, The fraction of cells with intact mitochondria was decreased after heat shock and the Δssa2 Δssa3 Δssa4 ssa1ts mutant showed more severe fragmentation and delayed recovery compared to the wild type (see also representative mitochondrial images in Fig. 3a). Shown are mean and s.e.m. quantified from three biological repeats with 1,335 cells for the wild type and 967 cells for the mutant. d, Representative images (from the 160-min time point of the plot in Fig. 3h) of ROS indicated by DHE signal. 1,621 mutant and 2,151 wild-type cells were imaged and quantified. Scale bars, 5 μm.
