Extended Data Figure 2: Radioligand-binding assays.
From: Structural basis for selectivity and diversity in angiotensin II receptors
a, b, Saturation binding of compound 1 and Sar1-Ile8-angiotensin II. Specific binding of [3H]compound 1 (a) and [125I]Sar1-Ile8-angiotensin II (b) to the wild-type (open circle) and engineered (closed triangle) AT2R, representative of two separate experiments. c–e, Competition binding of compound 1 (open circle), compound 2 (closed triangle) and angiotensin II (open square) to the wild-type AT2R (c), engineered AT2R (d) and wild-type AT1R (e) with [125I]Sar1-Ile8-angiotensin II as a tracer; each point represents the mean ± s.d. of two separate experiments, performed in duplicate.
