Extended Data Figure 7: Immune function assessment and molecular profiling of TCR diversity of rEC-HSC-derived T cells.
From: Conversion of adult endothelium to immunocompetent haematopoietic stem cells
a, CFSE dilution and intracellular interferon-γ (IFNγ) production upon CD45.2+ CD3/CD28 polyclonal activation and/or Treg addition. Representative flow cytometry plots. b, Quantification of intracellular IFNγ production upon CD3/CD28 polyclonal activation and/or Treg addition. Data represent mean ± s.e.m. (n = 5 independent experiments, 3 technical replicates); two-tailed unpaired t-test. c, Normalized counts for CD3+CD4+ cells. WBM control samples are denoted in blue, rEC-HSPC in green. d, Normalized counts for CD3+CD8+ cells. WBM control samples are denoted in blue, rEC-HSPC in green. e, Normalized counts for Jurkat cell samples. f, Analysis of TCR repertoire in Rag1−/− rEC-HSPC reconstituted mice upon chicken ovalbumin vaccination.
