Extended Data Figure 4: Fit of the model to the density.

a, Fit of fragments of the final model of the ABCG2 TMD to the post-processed and masked map from RELION. A region of up to 2 Å around the atoms is shown. The residue numbers are indicated, as are helices that are contained in these fragments and the EL3. b, Same as a but showing regions of 5D3-Fab. Residue numbers and whether the fragment belongs to the heavy or the light chain is indicated. c, Same as b but showing regions of the 5D3-Fab constant domain. d, Fit of the ABCG2 NBD model to the local-resolution-filtered map. A region of up to 3 Å around one ABCG2 NBD (including side chains) is shown. e, Same as a but showing the cholesterol (CHL) molecule. f, Fit of fragments of ABCG2 TM2, TM5a, and cholesterol to the post-processed and masked map from RELION. A region of up to 2 Å around the atoms is shown. g, Same as f but rotated by 180°.