Extended Data Table 2 Binding of [3H]-NNC0640 to membrane preparations from HEK293T cells expressing wild-type and mutant GCGRs

From: Structure of the full-length glucagon class B G-protein-coupled receptor

  1. ∗Values shown are means ± s.e.m. of at least three independent experiments.
  2. †Specific [3H]-NNC0640 binding (span) is defined as the window between the highest (no unlabelled ligand) and the lowest binding (unlabelled ligand at the highest concentration examined).
  3. ‡Protein expression levels of GCGR constructs in radioligand displacement studies are determined by flow cytometry with an anti-GCGR antibody and reported as per cent compared to wild-type (WT) GCGR.
  4. §If the span was <10% of the wild-type GCGR, IC50 is reported as no binding (NB).
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