Extended Data Figure 9: Comparison of object- versus pixel-based analysis (LLS).
From: Applying systems-level spectral imaging and analysis to reveal the organelle interactome
a, Comparison of object-based organelle contact analysis (bright) versus pixel-based organelle co-localization analysis (pastel). Object-based analysis data are the same as LLS data in Extended Data Fig. 8a. For the pixel-based analysis, a value of 1 indicates perfect co-localization, a value of 0 indicates the organelles are never co-located. No statistical test was performed. b, Half matrix showing pixel-based co-localization analysis for all the labelled organelle pairs, including those that were not included in the object-based analysis. a, b, n = 10 cells. The white line in the centre of each box represents the median value, the upper and lower edges of the boxes represent the 75th and 25th quantile of the data, respectively, and the upper and lower fences represent the 95% confidence level of the distribution.
