Extended Data Figure 2: Alignment of Cas9 protein sequences.
From: Structural basis of CRISPR–SpyCas9 inhibition by an anti-CRISPR protein
Multiple sequence alignment of the amino acid sequences of type II-A Cas9 proteins from Streptococcus pyogenes (GI 15675041), Listeria monocytogenes J0161 (GI 345535315), Listeria innocua Clip11262 (GI 16414891), and type II-C Cas9 proteins of Neisseria meningitidis (GI 518572566), Pasteurella multocida subsp. multocida str. Pm70 (GI 218767588), aligned using MUSCLE. Residues with more than 70% similarity are shown in red and boxed in blue. Residues involved in interaction with AcrIIA4 are indicated.
