Extended Data Figure 5: cDNA concentration of amplicon primer pools predicts sequencing outcome.
cDNA concentration of amplicon pools (as measured by Agilent 2200 Tapestation) is highly predictive of amplicon sequencing outcome. On each axis, 1 + primer pool concentration is plotted on a log scale. Each point is a technical replicate of a sample and colours denote observed sequencing outcome of the replicate. If a replicate is predicted to be passing when at least one primer pool concentration is ≥0.8 ng μl−1, then sensitivity is 98.71% and specificity is 90.34%. An accurate predictor of sequencing success early in the sample processing workflow can save resources.
