Extended Data Figure 7: Chronology of transcription factors expression during hPGC induction.
From: Principles of early human development and germ cell program from conserved model systems
a, Schematic of hPGC induction from pre-ME (12 h). b, Images of day 2 and 4 embryoids in response to BMP2 alone or BMP2 with LIF, SCF and EGF (cytokines). Notably, BMP2 alone can induce hPGC at almost the same efficiency as the full cytokines, but do not survive during extended culture, as shown previously. c, Immunostaining of embryoids induced with BMP2 alone or BMP2 with LIF, SCF and EGF showing expression of SOX17, BLIMP1 and TFAP2C. Scale bar, 50 μm. d, Proportion of SOX17+ cells indicated in Extended Data Fig. 7c. e, Immunostaining of embryoids induced with BMP2 alone or BMP2 with LIF, SCF and EGF showing expression of SOX17, BLIMP1 and NANOG. Scale bar, 50 μm. f, Proportion of SOX17+ cells in Extended Data Fig. 7e.
