Extended Data Figure 8: Effect of NANOG on hPGC induction, characterization of NANOS3–tdTomato reporter hPSCs containing inducible SOX17, BLIMP1 with or without TFAP2C, and similarity between cytokine- and SOX17–BLIMP1-induced hPGCs.

a, Represents overexpression of dex-inducible NANOG transgenes in NANOS3–tdTomato reporter comp-hPSC. b, Day 4 embryoids following induction of NANOG (by dex), with or without cytokines as indicated. c, FACS patterns after induction of hPGCs; NANOS3–tdTomato⁺AP⁺ cells (%) shown in Extended Data Fig. 8b. d, Represents overexpression of dex-inducible SOX17, dox-inducible BLIMP1, Shield1(S1)-inducible TFAP2C transgenes in NANOS3–tdTomato reporter comp-hPSCs. e, Immunostaining of NANOS3–tdTomato reporter comp-hPSCs + inducible SOX17/BLIMP1/TFAP2C (iSBT) 1 day after induction of SOX17, BLIMP1 and TFAP2C by addition of dex, dox or S1. Scale bar, 50 μm. f, Immunostaining of NANOS3–tdTomato reporter comp-hPSCs + iSB 1 day after induction of SOX17 or BLIMP1 by addition of dex or dox. Scale bar, 50 μm. g, Immunostaining of day 2 embyroid induced with or without dex to induce nuclear localization of SOX17. Notably, accumulation of SOX17 signal is observed in +dex condition. h, Changes in gene expression (RT-qPCR) during hPGC induction: comp-hPSCs control (AP⁺ cells); NANOS3–tdTomato⁺ hPGCs induced by SOX17–BLIMP1 or cytokines; NANOS3–tdTomato⁻ cells in cells exposed to cytokines (error bars, mean ± s.d.). i, Unsupervised hierarchical clustering (UHC) of gene expression. j, Gene set enrichment analysis (GSEA) of 123 hPGC-specific genes (Supplementary Table 1) on the transcriptome of cytokine- and SOX17–BLIMP1-induced hPGCs. k, Heat map showing expression of epigenetic modifiers related to global DNA demethylation. Same datasets as shown in Fig. 4e were used for analysis.

Source data