Extended Data Figure 10: Response of SOX17-knockout comp-hPSCs to SOX17 and BLIMP1, and changes in epigenetic modifier expression after overexpression of SOX17 and BLIMP1.
From: Principles of early human development and germ cell program from conserved model systems
a, Overexpression of dex-inducible SOX17 (iS) and dox-inducible BLIMP1 (iB) in SOX17-knockout comp-hPSCs. b, Immunostaining of comp-hPSC 1 day after induction of SOX17 (dex) or BLIMP1 (dox), or both, and day 2 embryoids following dex-induced SOX17 with endogenous or dox-induced BLIMP1. Scale bar, 50 μm. c, Gene expression (RT–qPCR) in day 4 embryoids following induction by dex (+SOX17), dox (+BLIMP1), or dex + dox (+SOX17 and BLIMP1). Bulk cells of embryoids induced from SOX17-knockout and wild-type comp-hPSCs with cytokines were used as controls. d, Upon specification, hPGCs become refractory to activin or Wnt signalling. Left schematic shows the experimental design. The embryoids were transferred to the medium with or without GSK3i (3 μM) or activin A (100 ng ml−1) at day 0, 1 or 2 to see the effect on PGC induction. FACS patterns (right) show the induction efficiency of hPGCs (percentage of NANOS3–tdTomato+AP+) at day 4. e, Day 4 embryoids induced by cytokines with or without activin A or GSK3i.
