Extended Data Figure 2: Proliferation and development of early pPGCs.
From: Principles of early human development and germ cell program from conserved model systems
a, OCT4 RNA in situ hybridization identifies the pPGC cluster (arrow) in the posterior end of E13.5–E15.5 embryos. Insets show whole embryos. b, Whole-mount OCT4 immunohistochemistry of a porcine embryo. Dashed square marks the area shown at higher magnification on the top right. Arrow points to the pPGC cluster. Bottom right: cross section of the embryo (line in the wholemount image) shows migratory pPGCs (red cells). c, Number of pPGCs at different stages as indicated. d, Immunostaining of EdU-labelled embryos at the indicated stages with different antibody combinations identifying the PGCs. The pPGC cluster is highlighted with dashed white line. Arrows show SOX17+EdU+ and SOX17+BLIMP1+EdU+ cells. Scale bar, 20 μm. e, Immunostained migratory pPGCs (arrows); inset show cells at higher magnification. f, Immunostained gonadal pPGCs. Inset shows SOX2+ neural tissue.
