Nature 502, 100–104 (2013); doi:10.1038/nature12519; corrigendum Nature 514, 654 (2014)

For the nested assay, designed mismatching of the outer reverse primer (SIVnestR01) and the codon-optimized portion of the vaccine gag insert sequence and specific priming of the reverse transcription step of the qRT–PCR assay is intended to prevent potential cross reactivity. Biological features of the RhCMV vectors used are expected to minimize the prospects of detection of vaccine insert-derived SIV gag RNA in plasma or insert-derived SIV gag DNA or RNA sequences in cell or tissue samples.

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