Extended Data Figure 6: Localization of and miRNA production from cytoplasmic viruses in diverse eukaryotes.
From: RNase III nucleases from diverse kingdoms serve as antiviral effectors
a, Zebrafish embryos were inoculated with SINV for 24 h and then analysed by FISH using a probe complementary to the capsid region of the genome. b, qPCR analysis of zebrafish embryos treated with the indicated morpholinos for 2 days (n = 4); error bars, s.d. c, A. thaliana protoplasts were mock-treated or TCV-infected for 40 h and then analysed by FISH using a Cy3-labelled probe complementary to bases 1210–1259 of the TCV genome. d, Quantification of mature miR-124 production from recombinant TCV was performed using the TaqMan miRNA assay on RNA from Fig. 4b. All samples were normalized to endogenous snoR66. Quantifications of each sample were performed in triplicate; error bars, s.d. from two biological replicates.
