Extended Data Figure 1: Characterization of RNaseIII−/− cells.
From: RNase III nucleases from diverse kingdoms serve as antiviral effectors
a, Sequence alignment of genetic alterations in the two alleles encoding Drosha in RNaseIII−/− cells. The deletion and insertion result in a frameshift and early stop codon. b, The ten most abundant miRNAs in each condition, parental HEK293T (WT) or RNaseIII−/− either mock-treated or SINV-infected for 24 h as determined by Illumina small RNA deep sequencing. c, Quantitative PCR (qPCR) analysis of DGCR8 mRNA levels in mock-treated and SINV-infected (MOI = 0.1, 8 h.p.i.) NoDice and RNaseIII−/− cells; error bars, s.d. from three independent experiments; all conditions had P < 0.05 by Student’s t-test. d, Transcriptome profiling and correlation analyses of NoDice cells at baseline and RNaseIII−/− cells transfected with GFP-tagged human Drosha for 72 h. Graph depicts data from two biological replicates per condition.
