Extended Data Figure 2: CG12721/Moonshiner is a germline-specific TFIIA-L paralogue.
From: A heterochromatin-dependent transcription machinery drives piRNA expression
a, Expression levels of indicated genes in larval/adult tissues on the basis of modENCODE RNA-seq data; rpkm, reads per kilobase per million mappers. b, The top schematic denotes the two regions of homology shown in Fig. 2a. Shown below is the amino-acid sequence alignment of these two regions from drosophilid species (Moonshiner) and selected insect species (TFIIA-L). The alignment was created using JalView with standard ClustalX colour coding and conservation score calculation. c, d, Western blot analyses of Flag–Moonshiner co-immunoprecipitation from lysates of S2 cells transfected with indicated expression constructs (IN, input; UB, unbound; IP, immunoprecipitate; asterisk, signal from anti-Flag heavy chain).
