Extended Data Figure 9: Appropriate control of PD-1 expression is important for Treg cells in suppressing TH2 immune responses.
From: Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signalling
a, b, Fold change of PD-1, GITR and OX40 expression on lung Treg cells (a), or splenic Treg cells (b) from OVA-sensitized C57BL/6 mice with or without OVA inhalation challenge (n = 3 each group). c, Expression of IL-4 in CD4+ T cells co-cultured with TSLP-DCs, or together with Treg cells transduced with control retrovirus (RV) or PD-1-expressing retrovirus (Pdcd1-RV). d, Expression of IL-4 in CD4+ T cells co-cultured with TSLP-DCs, or together with wild-type or PD-1-deficient Treg cells. e, Flow cytometry of eosinophils (CD11b+Siglec-F+) in the lung and spleen from Rag1–/– mice reconstituted with Scurfy:wild-type or Scurfy:Pdcd1–/– bone marrow cells. f, Concentration of IgG1 and IgG2a/c in the serum from the mice in (e) (n = 4 each group). Data are representative of two (a–f) independent experiments. Data are mean ± s.e.m. P values are determined by two-tailed Student’s t-test (a, b, f). NS, not significant; *P < 0.05, **P < 0.005. Numbers in gates indicate percentage of cells.
