Extended Data Figure 2: Excessive T cell activation and TH2-dominant inflammatory responses in Foxp3creStk11fl/fl mice.
From: Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signalling
a, Expression of CD62L and CD44 on CD4+ and CD8+ T cells in the spleen from wild-type and Foxp3creStk11fl/fl mice. b, Flow cytometry and quantification of IL-4- (WT, n = 4; Foxp3creStk11fl/fl, n = 5) and IL-5- (n = 4 each group) producing CD4+ T cells in the spleen from wild-type and Foxp3creStk11fl/fl mice (approximately 4–5 weeks old) after in vitro stimulation for 4 h. c, Flow cytometry of IL-4- and IL-5-producing Treg cells in the spleen from wild-type and Foxp3creStk11fl/fl mice. d, e, Flow cytometry and quantification of IFN-γ- (n = 4 each group; d) and IL-17-producing (WT, n = 4; Foxp3creStk11fl/fl, n = 5; e) CD4+ T cells in the spleen from the mice in b. f, Flow cytometry of IL-4-producing CD4+ T cells in the lung (left panel) and colon (right panel) from wild-type and Foxp3creStk11fl/fl mice. g, Expression of IL-5 and IL-17 in CD4+ T cells in the spleen from young wild-type and Foxp3creStk11fl/fl mice (approximately 16 days old). Right, fold changes of IL-5- or IL-17-producing CD4+ T cells from Foxp3creStk11fl/fl versus wild-type counterparts (n = 5 each group). Data are representative of at least three (a–g) independent experiments. Data are mean ± s.e.m. P values are determined by Mann–Whitney test (b, d, e) or two-tailed Student’s t-test (g). *P < 0.05, **P < 0.005, ***P < 0.0005. Numbers in quadrants or gates indicate percentage of cells.
