Extended Data Figure 4: LKB1 regulates the expression of distinct T_reg signature molecules.

a, Comparison of PD-1, GITR and OX40 expression on T_reg cells from wild-type and Foxp3^creStk11^fl/fl mice. b, Flow cytometry of resting (CD62L^hiCD44^low) and activated (CD62L^lowCD44^hi) T_reg cells in the spleen from wild-type and Foxp3^creStk11^fl/fl mice. c, Comparison of GATA3, IRF4, T-bet and RORγt in T_reg cells from wild-type and Foxp3^creStk11^fl/fl mice. d, Comparison of Foxp3, ICOS, CTLA-4 and CD25 expression in T_reg cells from wild-type and Foxp3^creStk11^fl/fl mice. e, Comparison of Foxp3, ICOS, CTLA-4 and CD25 expression in CD45.2⁺ wild-type and CD45.2⁺ Foxp3^creStk11^fl/fl T_reg cells from mixed bone marrow chimaeras. f, Expression of Stk11 mRNA in CD4⁺Foxp3–GFP⁺YFP⁺ T cells from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice (n = 7 each group). g, Flow cytometry of Foxp3–GFP⁺YFP⁺ T_reg cells from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice at day 20 after the last tamoxifen administration. h, Proportion of DAPI⁺ Foxp3–GFP⁺YFP⁺ T_reg cells in g. i, Flow cytometry of Foxp3–GFP⁺YFP⁺ T_reg cells from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice at day 6 after the last tamoxifen administration. j, k, Expression of IL-4 versus IL-13 (j) and IFN-γ versus IL-17 (k) in CD4⁺ T cells from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice after in vitro stimulation for 4 h. l, Comparison of Foxp3–GFP, ICOS and CD25 expression on Foxp3–GFP⁺YFP⁺ T_reg cells from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice. m, Flow cytometry of resting (CD62L^hiCD44^lo) and activated (CD62L^loCD44^hi) T_reg cells in the spleen from Foxp3^cre-ERT2Stk11^fl/+ and Foxp3^cre-ERT2Stk11^fl/fl mice. Data are representative of at least three (a–i, l, m) or two (j, k) independent experiments. Data are mean ± s.e.m. P values are determined by two-tailed Student’s t-test (f). ***P < 0.0001. Numbers above graphs indicate the mean fluorescence intensity; numbers in quadrants or gates indicate percentage of cells.

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