Extended Data Figure 5: LKB1 regulates Treg cell function independently of mTOR–HIF-1α axis or AMPK signalling.
From: Homeostatic control of metabolic and functional fitness of Treg cells by LKB1 signalling
a, b, Phosphorylation of S6 (a), and phosphorylation of AKT S473 and FOXO1 (b) in wild-type and Foxp3creStk11fl/fl Treg cells stimulated with or without anti-CD3/CD28 antibodies for 48 h. c, Flow cytometry of splenic Treg cells from wild-type, Foxp3creStk11fl/fl, Foxp3creHif1afl/fl and Foxp3creStk11fl/flHif1afl/fl mice. d, Expression of CD62L and CD44 on CD4+ T cells from the mice in c. e, Expression of IL-4 and IL-17 in CD4+ T cells from the mice in c after in vitro stimulation for 4 h. f, Phosphorylation of AMPK in wild-type and LKB1-deficient Treg cells stimulated with anti-CD3/CD28 antibodies for 48 h. g, Expression of CD62L and CD44 on CD4+ (upper) and CD8+ T cells (lower) from wild-type and Foxp3crePrkaa1fl/flPrkaa2fl/fl mice. h, Expression of IFN-γ, IL-4 and IL-17 in CD4+ (upper) and CD8+ T cells (lower) from wild-type and Foxp3crePrkaa1fl/flPrkaa2fl/fl mice after in vitro stimulation for 4 h. Data are representative of two (a–f) or three (g, h) independent experiments. Numbers in quadrants or gates indicate percentage of cells.
