Extended Data Figure 8: Analysis of Braf VE mice outside the central nervous system.
From: A somatic mutation in erythro-myeloid progenitors causes neurodegenerative disease
a, Proportion of YFP+F4/80+ cells in indicated organs analysed by flow cytometry. The proportion of YFP+ cells among F4/80+ cells from Braf WTcre+ (n = 6) was normalized and set to one (dotted line). Circles represent values for individual Braf VE mice (n = 7). Unpaired two-tailed t-test. b, Analysis of liver Kupffer cells as in a was performed on tissues from 5–8-month-old Braf VE (n = 5) and Braf WT (n = 4) mice on control diet, and 7–9-month-old Braf VE (n = 6) and Braf WT (n = 4) mice on PLX4720 diet. Circles represent values for individual mice. One-way ANOVA. *P < 0.05. c, Immunofluorescence analysis of pERK in F4/80+ Kupffer cells from 5–8-month-old Braf VE mice. Results are representative of n = 3. d, Serum analysis of Braf VE mice (score 1, n = 6) and littermate controls (n = 6). ALB, albumin; ALP, alkaline phosphatase; ALT, alanine aminotransferase; AST, aspartate aminotransferase. e, Gross liver, lung, kidney and spleen structure (haematoxylin and eosin, Trichrome) of paralyzed Braf VE and Braf WT mice. Representative of n = 7 mice per genotype. Scale bars, 200 μm and 10 μm (insets). f, Liver and spleen gross organs from paralyzed Braf VE and Braf WT mice. Representative of n = 5 mice per genotype.
