Extended Data Figure 4: Longitudinal study and PLX treatment of the Csf1r MeriCreMer;Braf V600E;Rosa26 LSL-YFP mice.
From: A somatic mutation in erythro-myeloid progenitors causes neurodegenerative disease
a, b, Latency to fall in the rotarod assay and footprint assay quantification for Braf VE mice (n = 7) and Braf WT littermates (n = 8). a, Rotarod assay at 1–4 months of age. Values are mean ± s.d. b, Rotarod and footprint assay at 4 months of age displaying single values. Mice that are score 1 are labelled in red. c, Footprint assay quantification of Braf VE mice at score 1 and littermate controls. Circles represent individual mice. n = 10 for Braf WT and n = 11 for Braf VE. d, Representative weight curves of Braf WT and Braf VE mice on control or PLX4720 diet. e, PLX4720 concentration in serum (ng ml−1), liver and brain (ng g−1) of 7–9-month-old Braf WT (n = 9) and Braf VE mice placed on the diet at 1 (n = 8) or at 3 months (n = 3) of age. Circles represent individual mice. f, Footprint assay quantification from Braf VE mice on PLX4720 diet at 1 month (n = 8) or at 3 months (n = 6) and control (Ctrl) diet (n = 13) and Braf WT (n = 32, black). Mice reaching paralysis were excluded from further analysis. See also g, where the dagger (†) indicates when Braf VE animals were euthanized. Values are mean ± s.d. Two-way ANOVA comparing treated and untreated Braf VE mice. *P < 0.05. g, Disease progression of Braf VE mice on control or PLX4720 diet. †Animal euthanasia owing to paralysis.
