Extended Data Figure 6: ILC2 are required for NMU-induced lung inflammation.

a, PBS or NMU was intranasally administered to C57BL/6 mice daily for four days. One day later, ILC2s from the lung were analysed by flow cytometry. Plots are gated on Lin^–CD45⁺GATA-3⁺CD25⁺ lymphocytes. b, PBS or NMU was delivered intranasally to C57BL/6 mice daily for five days. Three days later, eosinophil infiltration was determined in BAL by flow cytometry. Percentage (mean + s.d., n = 5) of CD11b⁺SiglecF⁺CD11c^– eosinophils in the BAL. c, PBS or NMU was intranasally administered to Rag2^−/− or Rag2^−/−Il2rg^−/− mice daily for four days. One day later, ILC2s and eosinophils from the lung were analysed by flow cytometry. d, PBS or NMU was intranasally administered daily for four days to Rag2^−/−Il2rg^−/− mice or Rag2^−/−Il2rg^−/− that were reconstituted with ILC2 progenitors. One day later, ILC2s and eosinophils from the lung were analysed by flow cytometry. e, PBS or NMU was intranasally administered to Nmur1^+/+ or Nmur1^−/− mice daily for four days. One day later, ILC2s and eosinophils from the lung were analysed by flow cytometry. Plots are gated on Lin⁻CD45⁺CD25⁺GATA-3⁺ lymphocytes. Data are representative of two (d, e) or three independent experiments (a–c) with similar results.

Source data