Extended Data Figure 2: MIA in SFB-absent Jax mothers does not induce changes in the total activity of the adult offspring, properties of the litter and maternal cytokine production.
From: Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring
a, b, Total investigation time (a) and total distance travelled (b) during the sociability test of adult offspring described in Fig. 2b–d. c, Litter size upon weaning (n = 59, 125 (Tac; PBS, poly(I:C)); n = 51, 50 (Jax; PBS, poly(I:C)); n = 55, 81 (co-housed Jax; PBS, poly(I:C)); n = 55, 89 (SFB-gavaged Jax; PBS, poly(I:C)). d, Weight of male offspring from the groups described in c (n = 32, 50 (Tac; PBS, poly(I:C)); n = 29, 27 (Jax; PBS, poly(I:C)); n = 29, 29 (co-housed Jax; PBS, poly(I:C)); n = 33, 30 (SFB-gavaged Jax; PBS, poly(I:C)). Data in c and d are from 7–8 independent experiments. e, f, Quantification of SATB2+ cells (e) in the cortex divided into ten equal bins representing different depth and of patch size (f) in the S1 (n = 4 (Tac; PBS); n = 3, 3, 4, 3 (Tac, Jax, co-housed Jax, SFB-gavaged Jax; poly(I:C)). g, Maternal plasma concentrations of TNFα and IFNβ at 3 h after PBS, poly(I:C) injection into Tac/Jax dams at E12.5; n = 4 per group. *P < 0.05, **P < 0.01, ***P < 0.001 calculated by two-way (e) and one-way ANOVA (a–d, f) with Tukey post hoc tests and Student’s t-test (g). Graphs indicate mean ± s.e.m.
