Extended Data Figure 3: SFB colonization leads to increased levels of gut TH17 cells in Jax pregnant mice.
From: Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring
a, Schematic of the experimental design. b, Flow cytometry of CD4+ T cells (gated on TCRβ+CD4+) stained intracellularly for IL-17a and RORγt. Mononuclear cells were collected at E14.5 from the ilea of poly(I:C)-treated Tac, Jax, co-housed Jax, SFB-gavaged Jax mothers. c, Representative s.e.m. images of epithelial surfaces in the ilea of Tac, Jax, co-housed Jax, SFB-gavaged Jax mothers. Scale bars, 30 μm. Data representative of 3 (b) and 2 (c) independent experiments. d, qPCR analysis for SFB levels in the faecal samples of the groups described in (a) (n = 4–5 per group). ****P < 0.0001 calculated by one-way (d) ANOVA with Tukey post hoc test. Graphs indicate mean ± s.e.m.
