Extended Data Figure 4: Poly(I:C)-induced inflammation during pregnancy, not after giving birth, is critical in inducing MIA-associated behavioural abnormalities in offspring.
From: Maternal gut bacteria promote neurodevelopmental abnormalities in mouse offspring
a, Schematic of the experimental design for cross-fostering experiments. b, USV index (n = 21, 20 (PBS dams; PBS, poly(I:C) pups); n = 22, 15 (poly(I:C) dams; PBS, poly(I:C) pups); 2–4 independent experiments). c–g, Marble burying index (c), time spent in the centre of an open field (d), and percentage of interaction (e), total investigation time (f), and total distance travelled (g) during the sociability test (n = 9, 14 (PBS dams; PBS, poly(I:C) pups); n = 12, 10 (poly(I:C) dams; PBS, poly(I:C) pups); 2 independent experiments). **P < 0.01, ****P < 0.0001 calculated by one-way (b–d, f, g) and two-way (e) ANOVA with Tukey post hoc tests. NS, not significant. Graphs indicate mean ± s.e.m.
