Extended Data Figure 8: Mfsd2b KO mice exhibited RBC phenotypes.
From: Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets
a, b, Reticulocytes (eight mice per genotype) and stomatocytes (9 WT and 13 KO) from 3- to 4-month-old male WT and KO mice. c, Scanning electron microscopy of RBCs from 7-month-old female WT and KO mice. We noted that stomatocyte phenotypes were progressive with age. d, e, KO RBCs had normal oxygen carrying capacity expressed as P50 values and normal deformability (11 mice per genotype, 2 experiments). f, Knockout mice had a significantly faster turnover rate than wild-type littermates (five mice per genotype). g, KO RBCs had a faster turnover rate in WT circulation (five mice per genotype). Reciprocal transfusion of CFSE-labelled WT or KO RBCs to KO or WT mice was performed. One day later, the mice were injected with 5FU to induce anaemia. CFSE-labelled RBCs were quantified by flow cytometry. h, Peripheral RBCs of five WT and four KO mice were enumerated at day 12 after 5FU treatment. ***P < 0.001, **P < 0.01, *P < 0.05, two-tailed unpaired t-test. Data are mean and s.d.
