Extended Data Figure 2: Reduction of S1P levels in HDL and albumin fractions of KO mice.
From: Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets
a, b, S1P levels from HDL and albumin fractions isolated from WT and KO mice. Each symbol represents one mouse (four WT, six KO). c, d, SDS gel and western blot analysis of HDL proteins from two independent isolation batches, respectively. One microlitre of HDL fraction from two representative mice for each isolation batch was diluted in SDS sample buffer and loaded and analysed on SDS gel or western blot probed with anti-ApoA1. The results showed that HDL isolated from batch 2 was not contaminated with albumin. These samples were used for the S1P analysis shown in a and b. ***P < 0.001, two-tailed unpaired t-test. Data are mean and s.d.
