Extended Data Figure 4: Postnatal deletion of Mfsd2b in RBCs reduces S1P export.
From: Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets
a, Genotyping of tail tips collected from WT, whole-body KO, and postnatal KO (Mfsd2b f/f) mice with or without Mx1-Cre. ‘Floxed’ denotes conditional alleles, WT denotes wild-type alleles, and KO denotes whole-body KO alleles. This shows that the Mfsd2b gene was still present in DNA samples extracted from the tails of the mice. b, Expression of Mfsd2b in RBCs was analysed by western blot collected from the indicated mice. Mfsd2b protein was barely visible after 3 weeks of treatment with poly(I:C) to induce Mx1-Cre in haematopoietic cells. Abbreviation ‘n.s.’ is non-specific protein band; Ter119 (glycophorin A) and β-actin antibodies were used as loading control. c, d, S1P export activity was strongly inhibited in RBC from Mfsd2b f/f; Mx1-cre mice. NS, not significant. ***P < 0.001, two-tailed unpaired t-test.
