Extended Data Figure 5: Mfsd2b KO mice exhibited a dramatic accumulation of S1P in platelets and mild splenomegaly.
From: Mfsd2b is essential for the sphingosine-1-phosphate export in erythrocytes and platelets
a, Total concentrations of S1P in platelets from male WT (seven mice) and KO mice (eight mice) (data were collected from two experiments). b, Platelet count in WT and KO blood (14 pairs, 3 experiments). c, d, Levels of S1P found in WT platelets and RBCs. Data are extracted from a and Fig. 4m for RBCs. e, Organ weights of WT and KO mice at 8 weeks old (five mice per genotype). Note that spleens of KO mice were significantly enlarged (11 mice per genotype for spleen weight). f, S1P export activity of nucleated cells isolated from the indicated tissues. S1P export was slightly increased in KO splenocytes (five biological replicates per genotype). ***P < 0.001, *P < 0.05, two-tailed unpaired t-test. Data are mean and s.d. (a–d) and s.e.m. (e, f).
