Extended Data Figure 7: Differential sensitivity of GBP family members to IpaH9.8-mediated degradation and translocation to S. flexneri.
From: Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence
a–c, HeLa cells stably expressing a GFP-tagged human (a, c) or mouse (b) GBP-family member were infected with mCherry-labelled S. flexneri (wild-type or ΔipaH9.8). a, b, Fluorescence images taken 2 h after infection (scale bar, 10 μm). c, Cells were primed with IFNγ, and cells containing GBP-decorated S. flexneri ΔipaH9.8 were counted. Approximately 200 infected cells were examined for each infection and data are expressed as mean percentages ± s.d. from three technical replicates. All data shown are representative of two independent experiments.
