Extended Data Figure 1: Rapid proteasomal degradation of hGBP1 by the T3SS of S. flexneri.
From: Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence
a, b, HeLa cells stably expressing GFP–hGBP1 and mCherry–Gal3 were infected with S. flexneri (wild-type or ΔmxiH mutant) or wild-type S. Typhimurium. Representative time-lapse images of GFP and mCherry fluorescence after infection (scale bar, 20 μm).Supplementary Videos 1 and 2 are corresponding videos for wild-type S. flexneri and S. Typhimurium infection, respectively. c, d, HeLa cells stably expressing GFP or GFP-hGBP1 were infected with S. flexneri or other indicated bacterial pathogens. Y. enterocolitica ΔHOPEM is a MRS40 strain-derived mutant deficient in five T3SS effectors. d, Infections were performed in the absence or presence of MG132 (50 μg ml−1). Cell lysates collected at indicated time points after infection were immunoblotted with GFP antibody. All data shown are representative of three independent experiments.
