Extended Data Figure 2: Transposon screen in S. flexneri identifies IpaH9.8, which mediates hGBP1 degradation; profiling of the specificity of anti-mGBP2 antibody.
From: Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence
a, The S. flexneri transposon screen for mutants defective for induction of hGBP1 degradation in HeLa cells stably expressing GFP–hGBP1 and mCherry–Gal3. b, Scatter plot of the fluorescence intensities of all the 96 wells in a representative plate (#103). The x and y axes show the intensity of mCherry and GFP fluorescence, respectively. G11 (red) is the no-infection control. The green points clustered with G11 are candidate hits. c, Fluorescence images of the candidate hits in b. d, Immunofluorescence with mGBP2 antibody. HeLa cells were transfected with the indicated GFP-tagged mGBP constructs. Cells were fixed 12 h after transfection. Scale bar, 10 μm. Data in c, d are representative of two independent experiments.
