Extended Data Figure 3: IpaH9.8 degrades hGBP1 more rapidly than NEMO, and is the only IpaH-family member that can degrade hGBP1.
From: Ubiquitination and degradation of GBPs by a Shigella effector to suppress host defence
a, b, Comparison of the degradation of endogenous hGBP1 and hGBP2 with that of NEMO by IpaH9.8. IFNγ-primed HeLa cells were infected with wild-type S. flexneri or ΔipaH9.8 mutant (a) or S. flexneri expressing IpaH9.8 or IpaH9.8-M3 (b). Cell lysates collected at indicated time points after start of the infection were immunoblotted as shown. c, HeLa cells stably expressing GFP–hGBP1 were transfected with the indicated mCherry–IpaH constructs. Fluorescence images were taken 24 h after transfection. Scale bar, 10 μm. d, IFNγ-primed HeLa cells were transfected with the indicated 6×Myc–IpaH constructs or IpaH9.8-C337A. Lysates were immunoblotted as shown. All data shown are representative of three independent experiments.
