Extended Data Figure 5: Staple lengths of more than 200 bases lead to decreased folding quality in the DNA origami nanorod.
a, Agarose-gel-electrophoretic analysis of self-assemly reactions of nanorods designed with staple lengths of 210 (left) or 420 (right) bases. Assembly mixtures were subjected to temperature ramps at different MgCl2 concentrations; 0 mM MgCl2 indicates reference samples that were not subjected to the temperature ramp. A well-folded structure would be expected to run faster than the scaffold in the reference sample. b, c, Negative-staining TEM micrographs of nanorods assembled using 210-base-long (b) or 420-base-long (c) staples.
